Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Organ biodistribution and intraoperative NIR FLI; ( A ) “Ex vivo” NIR FLI 6 h after 500 µM injection of RR11 and 23 ( MSA14 ) was performed to assess the compound accumulation in tumors and organs. ( B ) Representative intraoperative near-infrared (NIR), color and pseudo-colored fluorescent signal merge images of one 23 ( MSA14 ) injected subcutaneously engrafted OVCAR-3 mouse in vivo and ex vivo.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Ex Vivo, Injection, In Vivo

Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Biodistribution of RR11 and 23 ( MSA14 ) in SKOV-3 s.c. models. RR11 ( A ) and 23 ( MSA14 ) ( B ) were intravenously injected into n = 2 and n = 4 mice, respectively, and imaged at 2, 6, and 24 h with a dose of 500 and 100 µM. The fluorescence signal in the tumors (*), liver (#), limb (†), spine (‡) and background in the flank muscle (yellow square), is presented in one representative mouse. All animals were euthanized when indicated and “ex vivo” NIR FLI was performed. ( C ) 23 (MSA14) injected SKOV-3 mice were additionally imaged with the intraoperative imaging system “in vivo” and “ex vivo”. NIR exposure times are indicated in brackets.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Injection, Fluorescence, Ex Vivo, Imaging, In Vivo

Journal: Cancer

Article Title: Intraoperative Near-Infrared Fluorescence Imaging Targeting Folate Receptors Identifies Lung Cancer in a Large Animal Model

doi: 10.1002/cncr.30419

Figure Lengend Snippet: A.) Preoperative CT scan showing an 8 cm right-sided pulmonary adenocarcinoma (red arrow). First the surgeon used B.) white light imaging in vivo. Next C.) the tumor is fluorescent in vivo with a SBR of 4.6 using the fluoptics and D.) was reimaged using the Karl Storz NIR camera confirming fluorescence. E.) H&E shows pulmonary adenocarcinoma and F.) IHC demonstrates 3+ FR staining. Next, graphic representation of the mean tumor and background fluorescence of tumors imaged using the G.) Karl Storz and H.) Fuloroptics NIR imaging is shown.

Article Snippet: C.) NIR fluorescence image using the Karl Storz device.

Techniques: Computed Tomography, Imaging, In Vivo, Fluorescence, Staining

Journal: Cancer

Article Title: Intraoperative Near-Infrared Fluorescence Imaging Targeting Folate Receptors Identifies Lung Cancer in a Large Animal Model

doi: 10.1002/cncr.30419

Figure Lengend Snippet: A.) A preoperative CT scan showing a right-sided 5 cm lung tumor (red arrow). B.) A traditional white light ex vivo imaging of the specimen on the backtable. Yellow circles denote clock face and locations of both tumor margin and background fluorescence measurements. C.) NIR fluorescence image using the Karl Storz device. The tumor is circumscribed and brightly fluorescent with a mean SBR of 2.7. Note there was no available quantification of background fluorescence at 12 and 3 o’clock due to lack of surrounding normal lung. D.) Folate receptor IHC with 3+ FR tumor staining. E.) Fluorescence intensity decreases as distance from the tumor boarder increases. These measurements were obtained from tumors measured with the Karl Storz imaging system. The black bars represent tumor fluorescence directly at the tumor margin and the grey and white bars represent tumor fluorescence from the background (5 mm and 10 mm from the tumor edge, respectively).

Article Snippet: C.) NIR fluorescence image using the Karl Storz device.

Techniques: Computed Tomography, Ex Vivo, Imaging, Fluorescence, Staining

Journal: Cancer

Article Title: Intraoperative Near-Infrared Fluorescence Imaging Targeting Folate Receptors Identifies Lung Cancer in a Large Animal Model

doi: 10.1002/cncr.30419

Figure Lengend Snippet: Patient DR is a 55-year-old female with a mass in her left lower lobe noted on A.) preoperative CT scan which was confirmed pulmonary adenocarcinoma preoperatively. Images obtained intraoperatively include an B.) in vivo white light image, C.) NIR fluorescence image using the Karl Storz thoracoscopic system. Pathology confirmed D.) invasive adenocarcinoma on H&E. E.) IHC confirmed strong folate receptor staining.

Article Snippet: C.) NIR fluorescence image using the Karl Storz device.

Techniques: Computed Tomography, In Vivo, Fluorescence, Staining